Journal: International Journal of Cancer and Clinical Research

Article Title: Prdx Overexpression in Tumor Tissue of Breast Cancer Patients

doi: 10.23937/2378-3419/3/2/1053

Figure Lengend Snippet: Figure 1: Prdx Expression in Tumor (T) and Adjacent Normal (N) Tissue. (A) Oncopair INSTAblot IMB-130d, including matched tumor and adjacent non- tumor breast tissue for 7 patient donors. Tumor diagnosis for patients are as follows: P1, mucinous carcinoma; P2, mucinous carcinoma; P3, mucinous carcinoma; P4, metaplastic; P5, intraductal carcinoma; P6, intraductal carcinoma; P7, intraductal carcinoma; (B) Oncopair INSTAblot IMB-130a, including matched tumor and adjacent non -tumor breast tissue for 7 patient donors. All breast tumors were diagnosed as ductal carcinoma. (*note – the patient orientation of this blot is reversed from the published order, based on a difference in loading order for this blot lot).

Article Snippet: Two OncoPair INSTA-Blots membranes were purchased from Imgenex/Novus (San Diego, CA).

Techniques: Expressing, Biomarker Discovery

Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 1 | Decreased REV-ERBα protein abundance and increased protein levels of COL1A1 and LOX in IPF lungs compared to healthy control. Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc. Healthy controls contained 100% normal lung architecture with 85% alveoli surface area. IPF patient samples contained at least 50% lesion surface area. The protein abundance of REV-ERBα, COL1A1, and LOX were visualized and

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Quantitative Proteomics, Control

Journal: Nature communications

Article Title: Circadian clock molecule REV-ERBα regulates lung fibrotic progression through collagen stabilization.

doi: 10.1038/s41467-023-36896-0

Figure Lengend Snippet: Fig. 7 | IAV infection induced dysregulation of profibrotic progression exa- cerbated in Rev-erbα Het mice. WT and Rev-erbα Het mice (equal number of male and female) infected (103 PFU/mouse) with IAV for 15 days, and lungs were sepa- rated for RNA/protein isolation, or fixed with 10% formalin for FFPE sections. a The protein abundance of COL1A2, VIM and activated LOX were measured by western blot. Representative blot images were shown. Different targets were run on the same membrane: COL1A2, VIM and activated LOX were probed in the same membrane and β-ACTIN was used as an endogenous control (n = 5–6 mice per group). b The localizations of COL1A1 and LOX were determined by

Article Snippet: Compared to the control groups, the protein abundanceof REV-ERBαwas decreased in the healthy area (Control: 21.294%vs. healthy area from IPF: 11.296%) Fig. 1 | Decreased REV-ERBα protein abundance and increasedprotein levels of COL1A1 and LOX in IPF lungs compared to healthy control.Healthy control and IPF formalin fixed-paraffin embedded (FFPE) lung samples were purchased from Origene Inc.

Techniques: Infection, Isolation, Quantitative Proteomics, Western Blot, Membrane, Control

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 5. RAGE immunoblotting analysis of human intestine. SDS-PAGE of lung lysate (1.5 μg/lane) as well as lysates from small intestine (SI) and colon (50 μg/lane) was performed under reducing conditions. Membranes were stained with three different anti-RAGE antibodies; SC A11 (A), AF1145 (B), and AB D01P (C). Membranes were also stained with the respective secondary antibodies only (negative control). GAPDH was used as a loading control. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, SDS Page, Staining, Negative Control, Control

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 4. (A) RAGE immunoblotting analysis of human lung lysate. SDS-PAGE of lung lysate (1 μg/lane; PNGase F -) and deglycosylated lung lysate (1 μg/lane; PNGase F +) was performed under reducing conditions. Membranes were stained with four different anti-RAGE antibodies. Membranes were also stained with the respective secondary antibodies only (negative control). GAPDH was used as a loading control. (B) RAGE immunoblotting analysis of RAGE overexpressing (+) and control (−) HEK293T cells. SDS-PAGE was performed under reducing conditions. For WB analysis membranes were stained with anti-FLAG and anti-RAGE (AB D01P) antibodies. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, SDS Page, Staining, Negative Control, Control

Journal: Heliyon

Article Title: Human intestine and placenta exhibit tissue-specific expression of RAGE isoforms.

doi: 10.1016/j.heliyon.2023.e18247

Figure Lengend Snippet: Fig. 6. RAGE immunoblotting analysis of human placenta derived from different healthy (H) as well as GDM-, FGR- and PE-affected pregnancies. SDS-PAGE of lung lysate (1 μg/lane) as well as lysates from the placentas (25 μg/lane) was performed under reducing conditions. For WB analysis membranes were stained with three different anti-RAGE antibodies; SC A11 (A), AF1145 (B), and AB D01P (C). Membranes were also stained with the respective secondary antibodies only (negative control). Ponceau S staining was used as a loading control. The uncropped images are shown in the supplementary material of the manuscript.

Article Snippet: Human Lung Whole Tissue Lysate (Adult Whole Normal) was obtained from Novus Biologicals and protein extracts from healthy human small intestine and colon were obtained from Santa Cruz Biotechnology.

Techniques: Western Blot, Derivative Assay, SDS Page, Staining, Negative Control, Control

Journal: Hypertension

Article Title: Reduced Notch1 Cleavage Promotes the Development of Pulmonary Hypertension

doi: 10.1161/hypertensionaha.120.16065

Figure Lengend Snippet: Figure 3. Enhanced endothelial cell (EC) permeability and inflammation by Delta-like 4 neutralizing antibody (Dll4nAb) treatment in normal mice lung tissues. A, En face staining of N1-ICD (red) and VE-cadherin (green) on ECs of pulmonary arteries of Dll4nAb injected mice. Arrows, impaired adherent junction integrity (n=3 per group). Nuclei were counterstained by DAPI (blue). Scale bars, 10 µm. B, Quantification of trans- endothelial electrical resistance (TEER) and (C) paracellular permeability in confluent cultures of adult human pulmonary microvascular endothelial cell monolayers in the absence or presence of DAPT (10 µM) treatment for 12 h (n=4). (Continued )

Article Snippet: Normal lung tissue slides were purchased from Novus Biologicals (NBP2-30182).

Techniques: Permeability, Staining, Injection

Journal: Respiratory research

Article Title: Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis.

doi: 10.1186/s12931-023-02583-3

Figure Lengend Snippet: Fig. 1 ENO1 is upregulated in fibrotic lungs from human and bleomycin-treated mice. (A, B) IHC staining of ENO1 was performed in commercially avail able human normal (n = 3) and fibrosis (n = 3) lung FFPE sections. (C, D) After intratracheal injection of bleomycin (BLM, 3 mg/kg) (n = 7) or PBS vehicle control (Sham) (n = 4), the mouse lungs were harvested on day 21 for preparation of FFPE sections and subjected to IHC staining of ENO1. Representative pictures (A, C) and quantitative results of ENO1-stained positive areas were shown (B, D). After intratracheal injection of bleomycin (BLM, 3 mg/kg) (n = 4) or PBS vehicle control (Sham) (n = 4), the mouse lungs were harvested on day 21 for preparation of lysates and subjected to Western blotting for ENO1 (E) and the relative densitometry was shown below the representative blot after GAPDH normalization. Cropped blots were shown, and supplementary Fig. S1 presented the full-length blots. Quantitative results were shown by fold change after bleomycin treatment (F). Scale bar, 100 µM. *P < 0.05, **P < 0.01. (A, B) One experiment was performed and each picture or data point was from one human subject. (C-F) Data were representative for two independent experiments. Each picture, data point, or protein band was from one mouse except (F) was shown as mean ± SD

Article Snippet: Three human fibrotic lung FFPE sections (#CS701530, #CS702702, #CS703355) were obtained from OriGene (Rockville, MD, USA).

Techniques: Immunohistochemistry, Injection, Control, Staining, Western Blot

Journal: Respiratory research

Article Title: Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis.

doi: 10.1186/s12931-023-02583-3

Figure Lengend Snippet: Fig. 2 Blocking ENO1 ameliorates lung fibrosis in bleomycin-treated mice. After intratracheal injection of 3 mg/kg bleomycin (BLM) (day 0), mice were treated with ENO1 Ab HL217 (10 mg/kg) intravenously on a 6-day interval from day 1. Mouse lungs were harvested on day 14 or 21 for preparation of FFPE sections and subjected to H&E staining (A) and Masson’s trichrome staining (B). Representative pictures on day 21 (A, B) and quantitative results of Ashcroft score on day 21 (C) and inflammation score on day 14 (D) were shown. Body weight change (E), ratio of lung weight versus body weight (F), col lagen content of the lungs (G), and levels of TGF-β in BALF (H) were shown. Scale bar, 100 µM. *P < 0.05, **P < 0.01, ***P < 0.001. Data were representative for two independent experiments. Each picture or data point was from one mouse except (E) was shown as mean ± SEM

Article Snippet: Three human fibrotic lung FFPE sections (#CS701530, #CS702702, #CS703355) were obtained from OriGene (Rockville, MD, USA).

Techniques: Blocking Assay, Injection, Staining